Download Animal Cell Culture and Technology (THE BASICS (Garland by Michael Butler PDF

By Michael Butler

This ebook is a superb source for college kids and starting medical reaseraches. it's a stable resource of uncomplicated tools and strategies for winning laboratory mobile tradition strategies. many of the present telephone tradition tools are lined yet this insurance is not distinct. back, very good for rookies.

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Each cylinder has two regulator valves. One is a high-pressure gauge that measures the pressure in the cylinder with a range of 0–2000 psi and a second is a low-pressure gauge (0–30 psi) measuring the gas pressure into the incubator. Pressure tubing connects the low-pressure gauges of the two cylinders to a switching device, which if automated will switch the CO2 supply from cylinder one to cylinder two if the pressure falls below a certain level (typically 4 psi). 4). The % CO2 in the incubation chamber is controlled by a valve governed by intermittent readings of an infrared gas analyzer.

Myc and ras), which cause cell transformation. The retroviruses are also useful for incorporating recombinant DNA into animal cells (see Chapter 6). Alternatively, some cells can transform spontaneously in culture. This has been observed particularly with rodent cells and may be explained by the tendency of such cells to harbor endogenous viruses. 11. Cells from a culture collection For many applications, cell lines may be obtained from cell culture collections (′cell banks'), which have a large selection of well-characterized cell lines.

Aspirate with a pipette to ensure adequate suspension of the cells in the liquid. 8. Centrifuge at 100 g for 10 min and discard the supernatant. 9. Re-suspend the cell pellet and repeat steps 6 to 8 above. Notes The upper layer following the initial centrifugation contains blood plasma and is a good source of platelets. Washing the lymphocytes in salt solution ensures removal of the platelets. Granulocytes and erythrocytes form the lower layer after centrifugation. Typically this procedure recovers up to 80% of the lymphocytes from the original blood sample.

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